Oviductin sets the species-specificity of the mammalian zona pellucida
The zona pellucida (ZP) is vital for species-specific fertilization as this barrier mediates sperm-oocyte binding. Here, we determined whether sperm from distant mammalian orders (Carnivora, Primates, and Rodentia) could penetrate bovine oocytes by examining the role of bovine oviductal fluid and species-specific oviductal glycoprotein (OVGP1 or oviductin) from bovine, murine, or human sources in modulating the species-specificity of bovine and murine oocytes. Sperm from all the species were found to penetrate intact bovine ovarian oocytes to form hybrid embryos. However, contact with oviductal fluid or bovine, murine, or human OVGP1, conferred the ZP species-specificity, allowing only the penetration of the corresponding sperm regardless of the ZP's origin. Glycolytic and microstructural analyses revealed that OVGP1 covers the pores present in the ZP and that OVGP1 glycosylation determines sperm specificity. This suggests specific fertilization capacity is acquired in the oviduct through the ZP's incorporation of specific oviductin.
Authors
- Paula Cots
- Yulia Nathaly Cajas (ORCID: https://orcid.org/0000-0001-9791-6733)
- Manuel Avilés (ORCID: https://orcid.org/0000-0003-4396-4718)
- Karina Cañón-Beltrán (ORCID: https://orcid.org/0000-0002-0279-0857)
- Raúl Fernández‐González (ORCID: https://orcid.org/0000-0003-1989-2945)
- Dimitrios V. Rizos (ORCID: https://orcid.org/0000-0001-6813-3940)
- Juana M. Sánchez-Puig (ORCID: https://orcid.org/0000-0002-3966-3499)
- María Maroto (ORCID: https://orcid.org/0000-0001-8738-5932)
- Rafael Blasco (ORCID: https://orcid.org/0000-0002-8819-5767)
- Alfonso Gutiérrez‐Adán (ORCID: https://orcid.org/0000-0001-9893-9179)
- Ana Munoz‐Maceda (ORCID: https://orcid.org/0000-0001-6140-9238)
- Daniel de la Fuente (ORCID: https://orcid.org/0000-0001-9161-9076)
Institutions
- University of Cuenca (EC)
- Corporación Universitaria del Huila (CO)
- Instituto Murciano de Investigación Biosanitaria (ES)
- Institute for Animal Reproduction (JP)
- Universidad de Murcia (ES)
- Universidad Politécnica de Madrid (ES)
Publication Details
- Journal
- eLife
- Published
- 2024-10-31
- DOI
- https://doi.org/10.7554/elife.101338
- Primary Topic
- Plant and fungal interactions
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Comunidad de Madrid
- Centro de Biología Molecular Severo Ochoa
- European Commission
- Universidad de Murcia
- Agencia Estatal de Investigación