Cyclophilin A facilitates HIV-1 integration

Interaction between the HIV-1 capsid and host cellular factors is essential for infection. However, the molecular details and functional consequences of viral-host factor interactions during HIV-1 infection are not fully understood. Over 30 years ago, Cyclophilin A (CypA) was identified as the first host protein to bind to the HIV-1 capsid. Now it is established that CypA-capsid interaction promotes reverse transcription and nuclear entry of HIV-1. In addition, CypA blocks TRIM5α-mediated restriction of HIV-1. In this report, we show that CypA promotes the post-nuclear entry step of HIV-1 integration by binding to the viral capsid. Notably, we show that CypA stimulates the viral DNA integration activity of the HIV-1 preintegration complex. Collectively, our studies identify a novel role of CypA during the early steps of HIV-1 infection. This new knowledge is important because recent reports suggest that an operationally intact HIV-1 capsid enters the nucleus of an infected cell.

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Publication Details

Journal
Journal of Virology
Published
2024-10-31
DOI
https://doi.org/10.1128/jvi.00947-24
Citations
6
Primary Topic
HIV Research and Treatment
Type
article
Field-Weighted Citation Impact
1.76

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article

Cyclophilin A facilitates HIV-1 integration

Jiong Shi, Prem Prakash, Rajasree Chakraborty, Richa Dwivedi et al.
6 citations
Journal of Virology
HIV Research and Treatment
1.76
article

Cyclophilin A facilitates HIV-1 integration

Jiong Shi, Prem Prakash, Rajasree Chakraborty, Richa Dwivedi, Jui Pandhare, Adrian Padron, Chandravanu Dash, Jin-Woo Ahn, Christopher Aiken, Jeremy Luban, Kyusik Kim, Muthukumar Balasubramaniam
article en
6 citations

Abstract

Interaction between the HIV-1 capsid and host cellular factors is essential for infection. However, the molecular details and functional consequences of viral-host factor interactions during HIV-1 infection are not fully understood. Over 30 years ago, Cyclophilin A (CypA) was identified as the first host protein to bind to the HIV-1 capsid. Now it is established that CypA-capsid interaction promotes reverse transcription and nuclear entry of HIV-1. In addition, CypA blocks TRIM5α-mediated restriction of HIV-1. In this report, we show that CypA promotes the post-nuclear entry step of HIV-1 integration by binding to the viral capsid. Notably, we show that CypA stimulates the viral DNA integration activity of the HIV-1 preintegration complex. Collectively, our studies identify a novel role of CypA during the early steps of HIV-1 infection. This new knowledge is important because recent reports suggest that an operationally intact HIV-1 capsid enters the nucleus of an infected cell.

Journal of VirologyVol. 98(11)
Meharry Medical College (US), University of Massachusetts Chan Medical School (US), University of Pittsburgh (US), Vanderbilt University Medical Center (US)
National Institutes of Health
Good health and well-being
Openalex Percentile: Top 15%
HIV Research and Treatment
1.76
DOIOpen Access PDFOpenAlex
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