Extended Tool for Nanoparticles Incorporation Evaluation from Hyperspectral Images of Cultured Cells
Hyperspectral images of cultured cells incubated with mesoporous silica-based nanoparticles (NPs) functionalized with phenylboronic acid derivative and loaded with doxorubicin and resveratrol were analyzed to establish incorporation percentage. We designed and developed a digital tool, which extended the standard procedure, by combining spectral angle mapper with assisted segmentation and counting operations. It provides the percentage of pixels in which the spectral fingerprint of the NPs is found. The analysis is carried out at the cell level and points out the similarities and differences between cell lines and types of NPs.
Authors
- Ana Maria Pleava
- Roxana‐Cristina Popescu (ORCID: https://orcid.org/0000-0003-0815-8243)
- Mihaela Deaconu (ORCID: https://orcid.org/0000-0003-4775-8401)
- Diana Savu (ORCID: https://orcid.org/0000-0002-0145-5176)
- Mona Mihăilescu (ORCID: https://orcid.org/0000-0003-0337-8757)
- Daniela Berger (ORCID: https://orcid.org/0000-0002-7829-7540)
- Cosmin Cătălin Mustăciosu
- Simona Ioniţă (ORCID: https://orcid.org/0000-0001-8030-5397)
- Nicolae Tarbă (ORCID: https://orcid.org/0000-0002-7769-8289)
- Ionela Nicoleta Irimescu
- Eugen Scarlat
Institutions
- Universitatea Națională de Știință și Tehnologie Politehnica București (RO)
Publication Details
- Published
- 2024-10-09
- DOI
- https://doi.org/10.1109/cas62834.2024.10736785
- Primary Topic
- Spectroscopy and Chemometric Analyses
- Type
- article
- Field-Weighted Citation Impact
- 0.00