A dual strain probiotic administered via the waterline beneficially modulates the ileal and cecal microbiome, sIgA and acute phase protein levels, and growth performance of broilers during a dysbacteriosis challenge

Intestinal dysbacteriosis is increasing in broilers due to the reduced use of antibiotics in feed. This study tested the effect of daily waterline administration of a dual-strain probiotic comprising Lactobacillus acidophilus AG01 and Bifidobacterium animalis subspecies lactis AG02, on growth performance and intestinal health during a 3-step microbial challenge. In total, 900 Ross 308 males were assigned to 36 floor pens (25 birds/pen, 12 pens/treatment) in a completely randomized design. Birds were fed a corn, wheat and soybean-meal based diet. Diets were formulated in 3 phases (starter: 1 to 10; grower: 11 to 24; finisher: 25 to 42 d of age). Treatments comprised a non-challenged control (NC), challenged control (CC), and the CC supplemented with 1 × 10⁸ colony forming units (CFU)/bird/day of the probiotic (CC+Probiotic). The challenge comprised 1 × 10⁸ CFU/bird of Avian Pathogenic Escherichia coli on d 7, 4,000 oocysts/bird of Eimeria on d 15 and 1 × 10⁹ CFU/bird of C. perfringens on d 18, 19 and 20. Growth performance was monitored over 42 d, blood samples, and digesta were collected and intestinal dysbacteriosis scoring was performed. Compared to NC birds, CC birds exhibited reduced BW (all phases), reduced feed intake (starter and grower phase), increased FCR (grower phase and overall; P < 0.05), reduced ileal lactic acid bacteria concentrations (d 24 and 42), and increased cecal E. coli (d 24; P < 0.05). Compared to CC birds, CC+Probiotic birds exhibited increased BW, BW gain and feed intake during grower phase (P < 0.05), increased ileal lactic acid bacteria at d 24 and 42 and reduced ileal C. perfringens at d 24, increased mucosal secretory IgA and reduced serum alpha-1-acid-glycoprotein at d 42. The overall growth performance of CC+Probiotic birds was equivalent to NC birds. These results confirm the efficacy of the dual strain probiotic for mitigating the negative effects of a multi-microbial challenge, improving gut health and growth performance in commercial broilers under dysbacteriosis challenge.