Imaging flow cytometry reveals LPS-induced changes to intracellular intensity and distribution of α-synuclein in a TLR4-dependent manner in STC-1 cells
This study demonstrates that LPS-TLR4 signalling alters the intracellular localisation of α-synuclein in enteroendocrine cells in vitro and showcases the utility of combining imaging flow cytometry to investigate subtle protein changes that may not be apparent through confocal microscopy alone. Further investigation is required to understand the apparent dose-dependent effects of LPS on α-synuclein in the gut epithelium in healthy states as well as conditions such as Parkinson's disease.
Authors
- Chidozie C. Anyaegbu (ORCID: https://orcid.org/0000-0002-0618-3173)
- Melinda Fitzgerald (ORCID: https://orcid.org/0000-0002-4823-8179)
- Ryan S. Anderton (ORCID: https://orcid.org/0000-0003-0568-9794)
- Anastazja M. Gorecki (ORCID: https://orcid.org/0000-0001-8560-6666)
- Kathryn A. Fuller (ORCID: https://orcid.org/0000-0002-3462-7501)
Institutions
- The University of Western Australia (AU)
- Perron Institute for Neurological and Translational Science (AU)
- The University of Notre Dame Australia (AU)
Publication Details
- Journal
- Methods
- Published
- 2024-10-31
- DOI
- https://doi.org/10.1016/j.ymeth.2024.10.009
- Citations
- 8
- Primary Topic
- Parkinson's Disease Mechanisms and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 2.95
Funders
- Raine Medical Research Foundation
- University of Notre Dame Australia
- Pierce Armstrong Foundation
- Australian Research Council