Kinetics and Thermodynamic Study of Cellulase Embedded Metal Organic Frameworks

Cellulase is an important enzyme used for many purposes in different industrial sectors. Even though cellulase has so many applications, it easily denatures with a little change in pH and temperature, which causes low stability, usability, and activity. To enhance its activity and stability, immobilization of porous materials is the best way to enhance its activity, stability, and life span. For immobilization, a Metal-organic framework (MOF) is considered as a potential candidate. Cellulase@Zn- benzene 1-4 di-carboxylic acid (BDC) by hydrothermal method and Zn-cellulase-benzene 1-4 dicarboxylic acid (BDC) by de novo approach were prepared, and their activities were analyzed. Zn-cellulase-benzene 1-4 dicarboxylic acid (BDC) produced by the de novo approach, shows higher activity, stability, catalytic performance, and life span than the free enzyme, and cellulase@Zn- benzene 1-4 di-carboxylic acid (BDC) produced by the hydrothermal method.