Extracellular Recording of Electrical Activity in the <em>Drosophila</em> Central Nervous System

Source: Swale, D. R., et al. Electrophysiological Recording of The Central Nervous System Activity of Third-Instar Drosophila Melanogaster. J. Vis. Exp. (2018) The video demonstrates the extracellular recording of neuronal activity from the central nervous system (CNS) of Drosophila larvae. The transected CNS is positioned in a recording chamber, and the electrical activity from a descending nerve is recorded to establish the baseline firing frequency. A test agent is then introduced, and the change in firing frequency is recorded to determine the agent's excitatory or inhibitory effect.