Inhibition of Inflammation Response and the Immune-related Protein by Treatment of Actinidia arguta Pollen in Raw264.7 Macrophage

In this study, we proposed the functional effect of the anti-inflammation effect of the Actinidia arguta ( A.a.) bee pollen and the molecular mechanism of its anti-inflammation. Two A.a. bee pollen samples commercially available in Korea were extracted in 70% ethanol, and finally dissolved in 35% ethanol to prepare a 100 mg/mL. The A.a. bee pollen extracts was treated with LPS, an inflammatory factor, to Raw264.7 macrophages. The A.a. bee pollen extracts reduced the NO production induced by LPS by approximately 60%, indicating that the A.a. bee pollen extracts had an anti-inflammatory effect. In addition, the intracellular molecular mechanism of the anti-inflammatory effect of the A.a. bee pollen was confirmed by Western blotting, and the expression of inflammation-related proteins such as iNOS, COX-2, NF-κB, and IL-1β induced by LPS was reduced by the A.a. bee pollen. In addition, the expression of intracellular antioxidant molecules such as SOD2 was also decreased. However, it did not affect the phosphorylation and expression of MAPKs that related intracellular division and apoptosis. The results of this study showed that the molecular mechanism of the inhibitory effect of the A.a. bee pollen extract on hyperimmunity caused by inflammatory factors, thereby providing a basis for the immune function of the Korean A.a. bee pollen and the increased utilization of the pollen.