Establishment of the auxin inducible degron system for Babesia duncani: a conditional knockdown tool to study precise protein regulation in Babesia spp.
This study demonstrated the functionality of the AID system in regulating protein levels by inducing rapid degradation of eGFP using IAA, providing an important research tool for studying essential gene functions related to invasion, egress, and virulence of B. duncani. Moreover, it also offers a construction strategy for apicomplexan parasites that have not developed an AID system.
Authors
- Sihui Huang (ORCID: https://orcid.org/0000-0003-1178-2434)
- Wanxin Luo (ORCID: https://orcid.org/0000-0001-6887-3682)
- Yue He (ORCID: https://orcid.org/0000-0003-2105-5249)
- Xingai Guan
- Sen Wang (ORCID: https://orcid.org/0000-0001-7179-626X)
- Qi Zhang (ORCID: https://orcid.org/0009-0008-5984-6859)
- Dongfang Li (ORCID: https://orcid.org/0000-0001-8229-8258)
- Junlong Zhao (ORCID: https://orcid.org/0000-0001-5162-4495)
- Lan He (ORCID: https://orcid.org/0000-0002-5453-4425)
- Ya-Ting Zhou
- Qing Chen (ORCID: https://orcid.org/0009-0005-3886-0350)
Institutions
- Huazhong Agricultural University (CN)
- Hubei University (CN)
Publication Details
- Journal
- Parasites & Vectors
- Published
- 2024-10-31
- DOI
- https://doi.org/10.1186/s13071-024-06458-4
- Citations
- 1
- Primary Topic
- Plant Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.60
Funders
- National Natural Science Foundation of China
- National Key Research and Development Program of China
- Fundamental Research Funds for the Central Universities