Commercial Trade and Conservation: DNA Barcoding of Wild Reticulated Python (Malayopython reticulatus) (Schneider, 1801) for Sustainable Management

Abstract The reticulated python, scientifically known as Malayopython reticulatus , is a prominent member of the Pythonidae family, exhibiting a wide geographic distribution across Southeast Asia. Like most python species, it is listed on Appendix II of CITES, allowing for regulated harvest and commercial export. However, inadequate monitoring of snakeskin origins within Malaysia has facilitated illegal trade, exacerbating concerns over population declines. Thus, this study aims to document the DNA barcoding of M. reticulatus in Pantai Penyabong, Johor, utilizing the COI gene marker of mitochondrial DNA (mtDNA). Observations revealed 11 reticulated pythons, with two successfully captured for blood sample extraction and amplification. Sequences obtained exhibited a high identity percentage in the NCBI GenBank database, supported by similar topologies in phylogenetic trees. These findings demonstrate the efficacy of molecular identification techniques in combating illegal wildlife trade. Governments and law enforcement agencies can enforce conservation laws and protect wild populations by verifying mislabeled python species. This study highlights the crucial role of DNA barcoding in wildlife forensics and conservation management, offering a rapid and reliable method for species identification and monitoring in the face of escalating threats.