Comparison of IL-10, TGF-β1, and VEGF Levels in The Secretome of Limbus, Adipose, and Bone Marrow Mesenchymal Stem c3lls

Objective: In this study, we investigated whether the levels of IL-10, TGF-1, and VEGF in the secretome of limbal (LMSCs), adipose (AMSCs), and bone marrow (BMMSCs) mesenchymal stem cells are similar. Methods: LMSCs, AMSCs, and BMMSCs were isolated from a healthy 3-month-old New Zealand White Rabbit weighing 2.5–3.5 kg. The isolation of LMSCs from corneoscleral tissue was based on the protocol developed by Chen et al. and modified by Komaratih et al. Stem cell culture and secretome production were carried out according to the Stem Cell Research and Development Center, Universitas Airlangga protocol. Passage-5 MSCs were cultured using the hanging drop technique. MSCs are characterized using positive markers (α-SMA, CD-200, and Vimentin), while negative markers use CD-34 and CD-45. Examination of IL-10, TGF-b1, and VEGF levels in the secretome of LMSCs (LMSC-S), AMSCs (AMSC-S), and BMMSCs (BMMSC-S) using the ELISA method Results: The mean levels of IL-10 in the LMSC-S, AMSC-S, and BMMSC-S were 697.51 ± 57.12 ng/L, 692.94 ± 61.85 ng/L, and 645.73 ± 65.08 ng/L, there was no significant difference in IL-10 levels (r = 0.31). The mean levels of TGF-b1 in the three groups were 876.84 ± 70.75 pg/mL, 768.54 ± 38.12 pg/mL, and 798.82 ± 110.02 pg/mL, there was no significant difference in TGF-b1 levels (r = 0.62). The mean levels of VEGF in the three groups were 31.36 ± 3.95 ng/L, 31.93 ± 6.76 ng/L, and 34.33 ± 6.86 ng/L. There was no significant difference in VEGF levels (ρ= 0.63). Conclusions: The levels of IL-10, TGF-1, and VEGF in the LMSC-S, AMSC-S, and BMMSC-S were the same.