Identification Of Meloidogyne Species Associated With Tomato (Lycopersicon Esculentum L.) In The Bougouni Region (Mali)

Background: The development of market gardening and particularly that of tomatoes is confronted with multiple problems among which there are nematodes of the genus Meloidogyne. The determination of these parasites is important to adopt a control strategy. The objective of this study is to identify Meloidogyne species associated with tomato (Lycopersicon esculentum L.) in the Bougouni region of Mali. Materials and Methods: Root sample collection took place at two sites in Bougouni region. The roots were thinned with bleach 1%. The females were fixed on roots bearing galls in good condition, by the sodium hypochlorite-acid fuchsin method. These females were subsequently dissected and identified using the perineal plates. Results : Microscopic observation of the perineal plates revealed the presence of three types of perineal plates corresponding to M. incognita, M. arenaria and M. javanica with respective rates of 52%, 27% and 21%. Rootknot nematodes are very important in the parasitic nematofauna in Mali, they were found everywhere regardless of the site considered. Conclusion : These results confirm not only the omnipresence of these three genera in the intertropical zone but also the susceptibility of the Roma tomato to Meloidogyne.