Methods for Introdusing Fluorescent Labels into Polysaccharides
Polysaccharides are widely represented in plants and bacteria, where they are distinguished by their extraordinary structural diversity and the performance of various functions. To study the functions of polysaccharides, their fluorescent labeling is needed. This review discusses methods for introducing fluorescent labels into polysaccharides by chemical modification of certain functional groups of these complex biopolymers, as well as using the so-called bioorthogonal reactions, which allow labeling in a cell without affecting its viability. In addition to modification with organic dyes, the possibility of using quantum dots and coordination compounds of lanthanides is also discussed.
Authors
- Nicolai Vladimirovich Bovin (ORCID: https://orcid.org/0000-0001-8669-4477)
- Nadezhda Shilova (ORCID: https://orcid.org/0000-0001-6003-071X)
- Tatiana V. Ovchinnikova (ORCID: https://orcid.org/0000-0002-5950-249X)
- Alexey Nokel (ORCID: https://orcid.org/0000-0003-2903-2279)
- Alexander Borisovich Tuzikov (ORCID: https://orcid.org/0000-0001-5554-496X)
Institutions
- Institute of Bioorganic Chemistry (RU)
- National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov of the Ministry of Healthcare of the Russian Federation (RU)
Publication Details
- Journal
- Биоорганическая химия
- Published
- 2024-10-25
- DOI
- https://doi.org/10.31857/s0132342324040026
- Citations
- 1
- Primary Topic
- Click Chemistry and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.25