Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics
Live-cell imaging generally requires pretreatment with fluorophores to either monitor cellular functions or the dynamics of intracellular processes and structures. We have recently introduced full-field optical coherence tomography for the label-free live-cell imaging of fungi with potential clinical applications for the diagnosis of invasive fungal mold infections. While both the spatial resolution and technical set up of this technology are more likely designed for the histopathological analysis of tissue biopsies, there is to our knowledge no previous work reporting the use of a light interference-based optical technique for direct mycological examination and monitoring of intracellular processes. We describe the first application of dynamic full-field optical transmission tomography (D-FF-OTT) to achieve both high-resolution and live-cell imaging of fungi. First, D-FF-OTT allowed for the precise examination and identification of several elementary structures within a selection of fungal species commonly known to be responsible for invasive fungal infections such as <i>Candida albicans</i>, <i>Aspergillus fumigatus</i>, or <i>Rhizopus arrhizus</i>. Furthermore, D-FF-OTT revealed the intracellular trafficking of organelles and vesicles related to metabolic processes of living fungi, thus opening new perspectives in fast fungal infection diagnostics.
Authors
- Mathieu Blot (ORCID: https://orcid.org/0000-0001-6541-2434)
- Marc Sautour (ORCID: https://orcid.org/0000-0003-4550-9225)
- Louise Basmaciyan (ORCID: https://orcid.org/0000-0003-4214-8022)
- Olivier Thouvenin (ORCID: https://orcid.org/0000-0003-4853-7555)
- Pierre‐Emmanuel Charles (ORCID: https://orcid.org/0000-0002-0201-9544)
- Bianca Podac
- Eliott Teston (ORCID: https://orcid.org/0000-0002-6264-0607)
- Thomas Maldiney (ORCID: https://orcid.org/0000-0002-7514-2113)
- Fanny Lanternier (ORCID: https://orcid.org/0000-0003-3800-4683)
- Catherine Neuwirth (ORCID: https://orcid.org/0000-0003-0998-0669)
- Dea Garcia‐Hermoso (ORCID: https://orcid.org/0000-0001-5288-2904)
- Claude Boccara (ORCID: https://orcid.org/0000-0002-9603-0700)
- Christophe Guillet (ORCID: https://orcid.org/0000-0001-8104-4600)
- Jean‐Pierre Quenot (ORCID: https://orcid.org/0000-0003-2351-682X)
- Frédéric Dalle (ORCID: https://orcid.org/0000-0001-8849-7514)
- Marie‐Elisabeth Bougnoux (ORCID: https://orcid.org/0000-0003-4721-2740)
- Léa Boulnois
- Martine Boccara (ORCID: https://orcid.org/0000-0002-4620-7467)
- Nicolas Augey
- Abdellah Dighab
Institutions
- Hôpital Necker-Enfants Malades (FR)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Institut Pasteur (FR)
- Université de Bourgogne (FR)
- Arts et Métiers (FR)
- Université Paris Cité (FR)
- Université Paris Sciences et Lettres (FR)
- École Normale Supérieure - PSL (FR)
- Institut Langevin (FR)
- Assistance Publique – Hôpitaux de Paris (FR)
- Procédés Alimentaires et Microbiologiques (FR)
- Institut de Systématique, Évolution, Biodiversité (FR)
- Laboratoire Chrono-Environnement (FR)
- HESAM Université (FR)
- Centre de recherche Translationnelle en Médecine moléculaire (FR)
- Maison des Sciences sociales et des Humanités de Dijon (FR)
- Laboratoire d’Ingénierie des Systèmes Physiques et Numériques (FR)
- ESPCI Paris (FR)
Publication Details
- Journal
- Journal of Fungi
- Published
- 2024-10-26
- DOI
- https://doi.org/10.3390/jof10110741
- Citations
- 1
- Primary Topic
- Antifungal resistance and susceptibility
- Type
- article
- Field-Weighted Citation Impact
- 0.39