Transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in Panonychus citri

Citrus red mites (P. citri) are key pests affecting citrus production worldwide due to pesticide resistance. The resistance mechanisms of ten pesticides are known, but a comprehensive study using transcriptome data is missing. This study employed deeptools, cuffdiff, rmats, bcftools and other software to examine gene expression variation, alternative splicing (AS), and mutations in mite resistance. The research highlighted that pesticides can regulate gene transcription, and red mites with resistance increase cytochrome P450, glutathione S-transferases, carboxylesterase, and acetylcholinesterase expression. Pyridaben also induces new AS events. Fluazinam-induced mites show mRNA splicing peaking earlier than transcription, both peaking at one day and returning to baseline after two days. AS profiles are similar in different mite populations with overlapping pesticide resistances. Lastly, specific mitochondrial SNPs in mites might mediate resistance against select pesticides.

Authors

Institutions

Publication Details

Journal
Scientific Reports
Published
2024-10-28
DOI
https://doi.org/10.1038/s41598-024-74820-8
Citations
4
Primary Topic
Insect Pest Control Strategies
Type
article
Field-Weighted Citation Impact
2.40

Funders

Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in Panonychus citri

Xiaoyu Zheng, Li-Guang Tian, Xiaoqing Han, Panpan Wu et al.
4 citations
Scientific Reports
Insect Pest Control Strategies
2.40
article

Transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in Panonychus citri

Xiaoyu Zheng, Li-Guang Tian, Xiaoqing Han, Panpan Wu, Mengyuan Peng, Yidong Lv, Guohua Yu, Shuzhen Liu, Xinze Zhang, Chen Sun, Na Yang, Yi Zhang, Bin Liu, Meng Li, Shuchen Guo, Yanhong Ding, Yunlong Zhang
article en
4 citations

Abstract

Citrus red mites (P. citri) are key pests affecting citrus production worldwide due to pesticide resistance. The resistance mechanisms of ten pesticides are known, but a comprehensive study using transcriptome data is missing. This study employed deeptools, cuffdiff, rmats, bcftools and other software to examine gene expression variation, alternative splicing (AS), and mutations in mite resistance. The research highlighted that pesticides can regulate gene transcription, and red mites with resistance increase cytochrome P450, glutathione S-transferases, carboxylesterase, and acetylcholinesterase expression. Pyridaben also induces new AS events. Fluazinam-induced mites show mRNA splicing peaking earlier than transcription, both peaking at one day and returning to baseline after two days. AS profiles are similar in different mite populations with overlapping pesticide resistances. Lastly, specific mitochondrial SNPs in mites might mediate resistance against select pesticides.

Scientific ReportsVol. 14(1)
Weifang Medical University (CN), Nankai University (CN), Tianjin Economic-Technological Development Area (CN), Weifang People's Hospital (CN)
National Natural Science Foundation of China, Southwest University
Zero hunger
Openalex Percentile: Top 11%
Insect Pest Control Strategies
2.40
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.