Characterization and phylogenetic analysis of the chloroplast genome of <i>Diospyros</i> aff. <i>oleifera</i>

The <i>Diospyros</i> genus (Ebenaceae) has significant economic value. During field surveys, we discovered a <i>Diospyros</i> specimen showing morphological overlap with both <i>D</i>. <i>oleifera</i> and <i>D. kaki</i> var. silvestris, provisionally named <i>Diospyros</i> aff. <i>oleifera</i>. To resolve its taxonomy, we sequenced and analyzed its chloroplast genome. The complete chloroplast genome is 157,732 bp with a quadripartite structure. mVISTA analysis revealed unique sequence variations compared to related species. Phylogenetic analysis using 75 protein-coding genes grouped it with <i>D. oleifera</i>, indicating their close relationship. Our findings suggest this specimen likely represents a novel, undescribed species. This study provides insights into Diospyros diversity and a foundation for future research.