<i>In Vitro</i> Lipid Digestion of Milk Formula with Different Lipid Droplets: A Study on the Gastric Digestion Emulsion Structure and Lipid Release Pattern

In this study, the digestive properties of milk formulas (two concept milk formulas L1 and L2 with <i>D</i>_4,3 ∼5 μm and a control milk formula S1 with <i>D</i>_4,3 ∼0.5 μm) were evaluated using a dynamic digestion model simulating the infant gastrointestinal tract. The results showed that L1 and L2 had a lower lipolysis degree compared to S1 during gastric digestion and no significant difference at the end of the digestion process. Triacylglycerol lipolysis products were highly related to the lipid sources of milk formulas. At the end of digestion, glycerophospholipids in milk formulas were hydrolyzed to lysophospholipids (∼60-80%), while sphingomyelins were barely hydrolyzed. Concept milk formulas showed a complete spherical structure with a mean size of 3-5 μm during gastric digestion, while the control formula had large aggregates consisting of small lipid droplets. This study reveals that the structure of lipid droplets moderates the gastric digestion emulsion structure and further influences the digestive properties of milk formulas.