Landscape transcriptomic analysis of bovine follicular cells during key phases of ovarian follicular development
The transcriptome of ovarian follicular fluid cells had a predominance of granulosa cells in the dominant follicle at deviation, with upregulation of genes involved in cell viability, steroidogenesis, and apoptosis prevention, whereas in the non-selected follicle there was upregulation of cell death-related transcripts. Immune cell transcripts increased significantly after deviation, particularly in preovulatory follicles, indicating strong intrafollicular chemotactic activity. We inferred that immune cell invasion occurred despite an intact basal lamina, contributing to follicular maturation.
Authors
- Henry David Mogollón García
- João Carlos Pinheiro Ferreira (ORCID: https://orcid.org/0000-0002-7742-8254)
- Roberto Sartori (ORCID: https://orcid.org/0000-0001-6917-0057)
- Pedro Marcus Pereira Vidigal (ORCID: https://orcid.org/0000-0002-5116-9856)
- John P. Kastelic (ORCID: https://orcid.org/0000-0003-4607-3355)
- Milo C. Wiltbank (ORCID: https://orcid.org/0000-0001-8188-0991)
- Julián Camilo Ochoa
- Rodrigo de Andrade Ferrazza
- Flávia Florêncio de Athayde
Institutions
- Universidade Estadual de Londrina (BR)
- University of Wisconsin–Madison (US)
- Universidade Federal de Viçosa (BR)
- University of Calgary (CA)
- Universidade de São Paulo (BR)
- Universidade Estadual de Campinas (UNICAMP) (BR)
- Hospital Universitário da Universidade de São Paulo (BR)
- Universidade Estadual Paulista (Unesp) (BR)
Publication Details
- Journal
- Biological Research
- Published
- 2024-10-28
- DOI
- https://doi.org/10.1186/s40659-024-00558-2
- Citations
- 3
- Primary Topic
- Reproductive Physiology in Livestock
- Type
- article
- Field-Weighted Citation Impact
- 1.70
Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Conselho Nacional de Desenvolvimento Científico e Tecnológico