Rapid and sensitive detection of <i>Mycobacterium tuberculosis</i> using nested multi-enzyme isothermal rapid amplification in a single reaction
In this study, we have successfully developed a method called nested multi-enzyme isothermal rapid amplification (nestMIRA) for the detection of <i>Mycobacterium tuberculosis</i> (Mtb). This method involves a two-step thermostatic amplification process in the same tube and can be read using fluorescence and lateral flow dipstick (LFD) assays. It is known to be rapid, specific, and highly sensitive. Our method has shown promising results in the detection of clinical specimens, and we believe that it can be a valuable tool for the rapid detection of Mtb in a clinical setting.
Authors
- Yue Feng (ORCID: https://orcid.org/0000-0002-2718-0836)
- Xueshan Xia (ORCID: https://orcid.org/0000-0003-1120-8870)
- Qiaoling Yan
- Xinlin Wu
- Mi Zhang (ORCID: https://orcid.org/0000-0001-8180-2029)
- Cuixian Yang (ORCID: https://orcid.org/0000-0002-5266-5608)
- Gaowen Liu (ORCID: https://orcid.org/0000-0002-6520-5547)
- Yingchao Chang
- Li Liu
Institutions
- Kunming University of Science and Technology (CN)
- Kunming Medical University (CN)
- The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture (CN)
- Kai Biotech (South Korea) (KR)
- Yunnan Provincial Infectious Disease Hospital (CN)
- Dali University (CN)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2024-10-28
- DOI
- https://doi.org/10.1128/spectrum.00887-24
- Citations
- 3
- Primary Topic
- Biosensors and Analytical Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.55
Funders
- Kunming Medical University
- Recruitment Program of Global Experts