Salivary Extracellular Vesicles Separation: Analysis of Ultracentrifugation‐Based Protocols
The UC + PS and PBP + UC protocols demonstrate comparable efficiency in separating salivary EVs. However, the combined PBP + UC protocol, with its simplified processing capability, offers a significant advantage, particularly in the initial phase of EV separation. This finding suggests its potential application in clinical settings where time-sensitive simple processing is critical. Further validation is needed to confirm its effectiveness for transcriptomic and proteomic analyses.
Authors
- Ramos‐Godínez María del Pilar
- Gabriela Anaya‐Saavedra (ORCID: https://orcid.org/0000-0002-8881-867X)
- Castillejos‐García Itzel (ORCID: https://orcid.org/0000-0001-5318-3697)
- M Richter Eduardo
- Ramírez‐Amador Velia
- Mireya Cisneros-Villanueva (ORCID: https://orcid.org/0000-0002-5662-0247)
- Hidalgo‐Miranda Alfredo
Institutions
- Universidad Autónoma Metropolitana (MX)
- National Institute of Genomic Medicine (MX)
- Instituto Nacional de Cancerología (MX)
Publication Details
- Journal
- Oral Diseases
- Published
- 2024-10-27
- DOI
- https://doi.org/10.1111/odi.15171
- Citations
- 1
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- Consejo Nacional de Ciencia y Tecnología
- Universidad Autónoma Metropolitana
- Instituto Nacional de Medicina Genómica