Anti-neoplastic Effects of Juglans Regia L. Green Fruit Pericarp on Breast Cancer Cell Lines

Background: Recently, more attention has been paid to the use of complementary medicine in breast cancer (BC) treatment. Juglans green fruit pericarp was found to have anti-cancer characteristics. Objectives: We aimed here to assess the possible anti-proliferative effects of methanolic extract of Juglans green fruit pericarp in BC cell lines. Methods: MCF-7 and MDA-MB-231 cells were examined with different concentrations of Juglans green fruit pericarp extract. The cell viability was investigated using a 3 - 2, 5-diphenyl tetrazolium bromide (MTT) assay. Also, different phases of the cell cycle were analyzed utilizing a flow cytometry method. Finally, the ability of cancer cells to create colonies was studied post-treatment with Juglans green fruit pericarp. Results: Our findings showed that the methanol extract has substantial cytotoxicity on MCF-7 and MDA-MB-231 cell lines. We also observed a significant arrest in the G0/G1 phase in cancer cells post-treatment with the methanol extract. Conclusions: In summary, our results introduced a cytotoxic property for Juglans green fruit pericarp methanol extract for MCF-7 and MDA-MB-231 cell lines. Future studies are needed to further evaluate its utility as a potential complementary therapy for BC.