Studies on the effect of polyamines supplementation on growth and biochemical parameters in rose micropropagation

Rose (Rosa hybrida L. cv. bush rose) micropropagules were cultivated on MS medium supplemented with polyamines (PAs) [Cadaverine (Cad), Spermidine (Spd), and Putrescine (Put)] to investigate its effects on growth and multiplication under in vitro conditions during 2023. Polyamines were added to the medium at different concentrations of 10 mM, 30 mM, and 50 mM via filter sterilization before autoclaving, to assess their impact on in vitro growth parameters, which indicate that a lower concentration of polyamines, specifically at 30 mM, significantly enhances biomass accumulation and overall plant growth, whereas higher concentrations (50 mM) tend to exert a diminishing effect. Biochemical parameters revealed that polyamines at 30 mM notably increased the levels of key biomolecules, including carbohydrates content, proteins content, and chlorophylls content, and also shows significant level of phenol content in the rose micropropagules. Furthermore, antioxidant activities, as measured by superoxide dismutase and peroxidase activities, was markedly higher in micropropagules grown on medium supplemented with 30 mM polyamines These findings suggest that low concentrations of polyamines can serve as effective growth regulators, promoting enhanced growth and biochemical responses in rose micropropagation. Therefore, incorporating polyamines, particularly at optimal concentrations, could improve the efficiency and quality of rose micropropagation protocols, benefiting commercial production and conservation efforts.