Optimized method for fluorine-18 radiolabeling of Affibody molecules using RESCA
We present a general and optimized method for Al[<sup>18</sup>F]F labeling of RESCA-conjugated Affibody molecules, which can be widely applied to this class of peptide-based imaging agents. Moreover, radiochemical yields were improved when the labeling was conducted at 37 °C or above. In vitro and in vivo assessment of the respective tracers was promising, showing retained binding capacity as well as moderate defluorination, which is usually regarded as a potential downside for RESCA-conjugated tracers.
Authors
- Olivia Wegrzyniak (ORCID: https://orcid.org/0000-0001-9636-0390)
- Olof Eriksson (ORCID: https://orcid.org/0000-0002-2515-8790)
- Fredrik Y. Frejd (ORCID: https://orcid.org/0000-0002-6421-4142)
- Francesco Lechi
- John Löfblom (ORCID: https://orcid.org/0000-0001-9423-0541)
- Luke R. Odell (ORCID: https://orcid.org/0000-0001-7658-5103)
- Bo Zhang (ORCID: https://orcid.org/0000-0002-0393-8585)
- Jonas Eriksson
Institutions
- Uppsala University (SE)
- Uppsala University Hospital (SE)
- Science for Life Laboratory (SE)
- Affibody (Sweden) (SE)
- KTH Royal Institute of Technology (SE)
Publication Details
- Journal
- EJNMMI Radiopharmacy and Chemistry
- Published
- 2024-10-26
- DOI
- https://doi.org/10.1186/s41181-024-00304-9
- Citations
- 6
- Primary Topic
- Radiopharmaceutical Chemistry and Applications
- Type
- article
- Field-Weighted Citation Impact
- 2.43
Funders
- European Foundation for the Study of Diabetes
- Kungliga Tekniska Högskolan
- Vetenskapsrådet
- Barndiabetesfonden
- Uppsala Universitet
- Diabetesfonden
- Science for Life Laboratory