Comparison of DNA extraction procedures for detection of Mycoplasma bovis directly from extended bovine semen straw samples using a commercial M. bovis PCR
Under the experimental conditions applied, this rtPCR test efficiently detected M. bovis in extended bovine semen straw samples from DNA extracted using both semi-automated extraction platforms, irrespective of prior centrifugation of extended semen.
Authors
- Colin Birch (ORCID: https://orcid.org/0000-0002-4608-9093)
- G. O. Mayne
- Anne J. Ridley
- Alannah S. Deeney (ORCID: https://orcid.org/0000-0002-1465-0800)
- E. SHIRLEY TAYLOR
Institutions
- Animal and Plant Health Agency (GB)
Publication Details
- Journal
- BMC Veterinary Research
- Published
- 2024-10-26
- DOI
- https://doi.org/10.1186/s12917-024-04333-z
- Citations
- 1
- Primary Topic
- Microbial infections and disease research
- Type
- article
- Field-Weighted Citation Impact
- 0.34
Funders
- Department for Environment, Food and Rural Affairs, UK Government