Anti-Bacterial Potential of Multi-strain Probiotics Lactiplantibacillus plantarum (Strain Dad-13 and FNCC-0250) and Lacticaseibacillus paracasei GMRMP-001 against Escherichia coli FNCC-0091 In Vitro and In Vivo

Lactiplantibacillus plantarum Dad-13, Lactiplantibacillus plantarum FNCC-0250 and Lacticaseibacillus paracasei GMRMP-001 are probiotics strain isolated from Indonesian local and traditional fermented foods. This study aims to evaluate the administration of multi-strain probiotics at dose of 109 CFU/mL/day for 14 days against the diarrhea-causing bacteria Escherichia coli FNCC-0091 at a dose of 1011 CFU/mL/day. Thirty rats were randomized and the divided into 5 groups, including control group, skim milk group, single-strain group, multi-strain group and E. coli group. Antibacterial activity was analyzed in vitro, feed intake, body weight were analyzed, microbial analysis was carried out on feces and cecum, short chain fatty acids were analyzed and intestinal morphology was measured. The result showed that the single-strain and multi-strain inhibition zone in in vitro test produces a very strong inhibitory potential (> 9 mm). The number of L. plantarum and L. paracasei increased and the number of E. coli decreased significantly in the feces and cecum of Rat indicating the survival rate of the probiotic bacteria in the digestive tract and their ability to fight pathogenic bacteria. The concentration of acetic acid, propionic acid and butyric acid compounds also increased, indicating that the digestive tract is at a low pH so that it can inhibit the growth of pathogenic bacteria in rats. In addition, consumption of multi-strain probiotics does not have a negative effect on intestinal morphology. The conclusion is that multi-strain probiotics are able to inhibit pathogenic bacteria in vivo and in vitro. HIGHLIGHTS Multi-strain probiotics isolated from traditional Indonesian fermented foods can reduce the potential for diarrhea. Single-strain and multi-strain have the same effectiveness in inhibiting pathogenic bacteria as seen from the growth of the bacteria. Digestive conditions were damaged in rat that were not given probiotic intervention, but when they received probiotic intervention, digestive conditions improved. GRAPHICAL ABSTRACT