Strategies to verify equimolar peptide release in mass spectrometry-based protein quantification exemplified for apolipoprotein(a)
These data further corroborate the validity of our apo(a) candidate RMP as higher order method for certification of commercial Lp(a) tests that is endorsed by the International Federation of Clinical Chemistry and Laboratory Medicine. MS-based molecular detection and quantification of heterogeneous apo(a) proteoforms will allow manufacturers' transitioning from confounded lipoprotein(a) [Lp(a)] mass levels into accurate molar apo(a) levels.
Authors
- Yuri E. M. van der Burgt (ORCID: https://orcid.org/0000-0003-0556-5564)
- Christa M. Cobbaert (ORCID: https://orcid.org/0000-0003-3565-1404)
- Maxim M. Treep (ORCID: https://orcid.org/0000-0002-0725-608X)
- Fred P.H.T.M. Romijn (ORCID: https://orcid.org/0000-0003-4470-7959)
- L. Renee Ruhaak (ORCID: https://orcid.org/0000-0003-3737-3807)
Institutions
- Leiden University Medical Center (NL)
Publication Details
- Journal
- Clinical Chemistry and Laboratory Medicine (CCLM)
- Published
- 2024-10-25
- DOI
- https://doi.org/10.1515/cclm-2024-0539
- Primary Topic
- Advanced Proteomics Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Leids Universitair Medisch Centrum