The role of herpesviruses in endometrial pathology: frequency of herpesvirus DNA detection in the endometrium, cervix and vagina in patients with chronic endometritis and endometrial hyperplasia

Introduction. Chronic endometritis (CE) is reported in 12% of patients with primary infertility. Herpesviruses are considered as one of the causes of CE. The aim of the study. To evaluate the frequency of herpesviruses DNA detection in endometrium, cervix and vagina in patients with chronic endometritis and endometrial hyperplasia. Materials and methods. 167 women of reproductive age were included in the study. Samples from the posterolateral wall of the vagina, cervix and endometrium were taken from each patient. The obtained samples were analyzed by real-time PCR for the presence of Herpes simplex viruses type 1 and 2 (HPV 1,2), Epstein-Barr virus (EBV), Cytomegalovirus (CMV) and Human herpesvirus type 6 (HHV6). Endometrial tissue was subjected to morphologic examination. Results. Depending on the morphology of endometrial biopsy, all patients were divided into three groups: with CE (N=53), with endometrial hyperplasia (EH, N=63), with normal endometrium (N=51). There were no statistically significant differences in the frequency of herpesvirus detection in endometrial, cervical, and vaginal samples. In endometrial samples, EBV was detected in 1 (1.9%) patient with CE (1.9%) and 1 (1.6%) patient with EH, HPV1,2 — in 1 (2%) woman with normal endometrial histology; DNA of other herpesviruses was not detected. Conclusions. The relationship between herpesvirus type, positive locus and the presence of endometrial pathology has not been established. The frequency of herpesvirus DNA detection in endometrial, cervical, and vaginal samples was not significantly different between the groups of women with CE, EH, and normal endometrial histology. Only 2 (1.7%) of endometrial samples with pathologic morphology were positive for DNA of herpesviruses, in both cases it was EBV.