A novel in vitro shoot excision technique for enhancing proliferation in banana cv. Chini Champa

Micropropagation technology has been commercially exploited for mass multiplication of banana. Several parameters such as nutrient media, explants, culture conditions etc have been standardized. However, a novel in vitro shoot excision technique designed to enhance the proliferation rate of banana cv. Chini Champa has been examined for the first time. We meticulously examined the impact of excision angles (45º and 90º) and excision site (Tip, middle and base) during shoot proliferation stage. Our findings unequivocally demonstrate that employing a 45º angle excision and tip excision yield the highest multiplication rates and biomass accumulation, surpassing other excision angles and methods. The substantial enhancement in shoot numbers, growth and biomass underscores the potential of this technique for improving banana propagation protocols, offering a valuable tool for sustainable banana production.