Assessing the criterion validity of the activPAL CREA v1.3 algorithm and ActiPASS 2023.12 software for detecting steps during a progressive treadmill-based laboratory protocol

Thigh-worn accelerometry is commonly implemented to measure step cadence. The default activPAL CREA algorithm is a valid measure of cadence during walking, but its validity during running is unknown. The ActiPASS software is designed to analyse tri-axial accelerometry data from various brands. We tested the validity of CREA v1.3 and ActiPASS 2023.12 to measure step cadence against manually-counted steps. Forty-five participants (26♀, 23.4 ± 4.6 years) completed 5 walking (6 min each, 2-4 mph) and 5 running treadmill (5-7 mph) stages (423 total stages completed). Based on equivalence testing, walking cadence (stages 1-5: 92-124 steps/min) from CREA was statistically equivalent (zone: <±2.2% of the manually-counted mean) to manual counts (92-125 steps/min). However, CREA underpredicted cadence during running stages (stages 6-10: 143-135 steps/min) by ~ 11-20 steps/min (<i>p</i> < 0.001). The ActiPASS-derived cadences were equivalent (zone: <±3.3%) to manual counts for all walking stages (99-127 steps/min) except Stage 1 (zone: ±10.5%). ActiPASS underpredicted cadences during running (stages 6-10: 137-153 steps/min) by ~ 10-16 steps/min (<i>p</i> < 0.001) compared to manual counts (stages 6-10: 154-164 steps/min). The CREA v1.3 algorithm is a valid measure of cadence during walking while ActiPASS 2023.12 is a valid measure of cadence during medium-fast walking. Further research is required to improve step cadence estimation across ambulation speeds.