Efficient gene disruption in polyploid genome by Cas9–Trex2 fusion protein
The fusion of the exonuclease Trex2 with the Cas9 protein significantly enhanced the efficiency of genome editing in hexaploid common wheat, particularly for the simultaneous editing of multiple favorable alleles within a single generation, thereby facilitating genome editing-assisted breeding in polyploid crops.
Authors
- Xing Wang Deng (ORCID: https://orcid.org/0000-0001-8709-1467)
- De Niu (ORCID: https://orcid.org/0000-0002-4745-3352)
- YaoYao Zhu
- Wenjing Sun (ORCID: https://orcid.org/0009-0003-8384-5665)
- Wenbo Pan (ORCID: https://orcid.org/0000-0001-9734-3090)
- Yuehui He (ORCID: https://orcid.org/0000-0003-3304-4555)
- Huawei Zhang (ORCID: https://orcid.org/0000-0002-8893-1429)
- Qi Xie (ORCID: https://orcid.org/0000-0002-8262-9093)
- Qiang Long
- Jiao Zhang
- Jian Li (ORCID: https://orcid.org/0009-0008-6910-5712)
- Xiying Yan
- Jinghua Cheng (ORCID: https://orcid.org/0009-0006-6964-1211)
- Chunlei Gao
Institutions
- South China Normal University (CN)
- Chinese Academy of Sciences (CN)
- State Key Laboratory of Plant Genomics (CN)
- Institute of Genetics and Developmental Biology (CN)
Publication Details
- Journal
- Journal of Integrative Plant Biology
- Published
- 2024-10-25
- DOI
- https://doi.org/10.1111/jipb.13797
- Citations
- 5
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 1.17
Funders
- Taishan Scholar Foundation of Shandong Province
- Natural Science Foundation of Shandong Province