LncBNIP3 Inhibits Bovine Intramuscular Preadipocyte Differentiation via the PI3K-Akt and PPAR Signaling Pathways
Intramuscular fat (IMF) content is an economic trait in beef cattle that improves the meat quality. Studies have highlighted the correlation between long noncoding RNAs (lncRNAs) and IMF development. In this study, lncBNIP3 knockdown promoted bovine intramuscular preadipocyte differentiation. RNA-seq analysis of intramuscular preadipocytes with lncBNIP3 knockdown identified 230 differentially expressed genes. The PI3K-Akt and PPAR signaling pathways were enriched. lncBNIP3 interference promoted mRNA and protein expression of key genes in PI3K-Akt signaling pathway. LncBNIP3 interference reversed the effects of an AKT-inhibitor MK-2206 on Akt protein expression and lipid droplet accumulation, promoted mRNA and protein expression of essential genes in the PPAR signaling pathway, and ameliorated the inhibitory effects of a PPARg antagonist GW9662 on lipid accumulation. Therefore, lncBNIP3 inhibition of bovine intramuscular preadipocyte differentiation is likely mediated via the PI3K-Akt and PPAR signaling pathways. This study identified a valuable lncRNA with functional roles in IMF accumulation and revealed new strategies to improve beef quality.
Authors
- Sayed Haidar Abbas Raza (ORCID: https://orcid.org/0000-0002-0961-1911)
- Rajwali Khan (ORCID: https://orcid.org/0000-0001-5191-3457)
- Bandar Hamad Aloufi (ORCID: https://orcid.org/0000-0003-3387-9034)
- Gongwei Zhang (ORCID: https://orcid.org/0000-0002-9043-6945)
- Fuyuan Zuo (ORCID: https://orcid.org/0000-0003-1612-6743)
- Linsen Zan (ORCID: https://orcid.org/0000-0002-2645-0179)
- Wucai Yang (ORCID: https://orcid.org/0000-0003-0708-3032)
- Bing‐Zhi Li (ORCID: https://orcid.org/0000-0003-4121-3048)
- Wenzhen Zhang (ORCID: https://orcid.org/0000-0003-3368-857X)
Institutions
- South China Agricultural University (CN)
- Southwest University (CN)
- The University of Agriculture, Peshawar (PK)
- Kerala Veterinary and Animal Sciences University (IN)
- University of Ha'il (SA)
- Key Laboratory of Guangdong Province (CN)
- Livestock Breeding and Veterinary Department (MM)
- Department of Animal Health (VN)
- Yangzhou Vocational University (CN)
- National Livestock Breeding Center (JP)
- Northwest A&F University (CN)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2024-10-25
- DOI
- https://doi.org/10.1021/acs.jafc.4c05383
- Citations
- 16
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 3.85
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Chongqing
- Fundamental Research Funds for the Central Universities