The effect of human umbilical cord mesenchymal stem cells combined with concentrated growth factor on repairing necrotic pulp caused by dental caries
This study investigated the impact of combining human umbilical cord mesenchymal stem cells (hUC-MSCs) with concentrated growth factor (CGF) on regenerating necrotic pulp. Ten-month-old male Bama miniature pigs were divided into control and caries groups. The experimental teeth were randomly divided into three groups: caries untreated, Ca(OH)<sub>2</sub>, and engineering dental pulp-like tissue (EDPT). hUC-MSCs and CGF scaffold were combined to construct EDPT, and the histological structure was observed. Odontoblasts and dental pulp cells were counted in each group. The results showed that hUC-MSCs adhered firmly to the porous mesh CGF scaffold, grew vigorously, and stretched sufficiently. In the EDPT group, odontoblasts in the root canal were arranged neatly, and predentin was formed. The odontoblast and dental pulp cell counts in the EDPT group were statistically significant compared to the caries untreated and Ca(OH)<sub>2</sub> groups. The hUC-MSCs-CGF could successfully repair necrotic pulp in animals with dental caries.
Authors
- Siyu Liu (ORCID: https://orcid.org/0000-0001-7831-5983)
- Jie Wang (ORCID: https://orcid.org/0000-0001-7870-0646)
- Feifei Wang (ORCID: https://orcid.org/0000-0002-2351-6873)
- Lijie Zhang (ORCID: https://orcid.org/0000-0002-8499-1029)
- Shifeng Fan
Institutions
- Hebei Medical University (CN)
- Third Hospital of Hebei Medical University (CN)
- Tangshan People's Hospital (CN)
Publication Details
- Journal
- Dental Materials Journal
- Published
- 2024-10-25
- DOI
- https://doi.org/10.4012/dmj.2024-007
- Primary Topic
- Mesenchymal stem cell research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Health Commission of Hebei Province