New Records and New Species of Dacnusini (Hymenoptera: Braconidae, Alysiinae) Based on Morphological and Molecular Evidence

Dacnusini is a species-rich tribe in the subfamily Alysiinae, with most species exclusively serving as parasitoids of leaf-mining Diptera (Agromyzidae). The number of genera discovered in China remains limited, which is apparently insufficient considering the global diversity of species and genera within this tribe, particularly given the vast and ecologically diverse landscapes of China. In the present study, three new record genera, <i>Victorovita</i> Tobias, <i>Coloneura</i> Foerster, and <i>Laotris</i> Nixon, were documented for the first time in China. In addition, the species delimitation approach and haplotype network analyses based on the COI sequences, combined with morphological evidence, were employed to delimit species. The findings indicated three new species: <i>Laotris glabella</i> sp. nov., <i>Laotris aethidentata</i> sp. nov., and <i>Victorovita aequalis</i> sp. nov. Additionally, K2P divergences showed no overlap between intra- and interspecific genetic distances in the <i>Laotris</i> and <i>Victorovita</i> species. Detailed descriptions for new species and keys to the species of <i>Laotris</i> and <i>Victorovita</i> are provided in this paper, along with the documentation of two new species records for China: <i>Victorovita caudata</i> (Szépligeti, 1901) and <i>Coloneura stylata</i> Foerster, 1863.