Insights into chemoautotrophic traits of a prevalent bacterial phylum CSP1-3, herein <i>Sysuimicrobiota</i>
Candidate bacterial phylum CSP1-3 has not been cultivated and is poorly understood. Here, we analyzed 112 CSP1-3 metagenome-assembled genomes and showed they are likely facultative anaerobes, with 3 of 5 families encoding autotrophy through the reductive glycine pathway (RGP), Wood-Ljungdahl pathway (WLP) or Calvin-Benson-Bassham (CBB), with hydrogen or sulfide as electron donors. Chemoautotrophic enrichments from hot spring sediments and fluorescence <i>in situ</i> hybridization revealed enrichment of six CSP1-3 genera, and both transcribed genes and DNA-stable isotope probing were consistent with proposed chemoautotrophic metabolisms. Ancestral state reconstructions showed that the ancestors of phylum CSP1-3 may have been acetogens that were autotrophic via the RGP, whereas the WLP and CBB were acquired by horizontal gene transfer. Our results reveal that CSP1-3 is a widely distributed phylum with the potential to contribute to the cycling of carbon, sulfur and nitrogen. The name <i>Sysuimicrobiota</i> phy. nov. is proposed.
Authors
- Yu-Zhen Ming (ORCID: https://orcid.org/0000-0003-2330-5117)
- Zheng‐Shuang Hua (ORCID: https://orcid.org/0000-0002-2405-4228)
- Ze‐Tao Liu
- LV Ai-ping
- Wensheng Shu
- Nimaichand Salam (ORCID: https://orcid.org/0000-0002-8834-8911)
- Wen-Jun Li (ORCID: https://orcid.org/0000-0002-4237-6140)
- Brian P. Hedlund (ORCID: https://orcid.org/0000-0001-8530-0448)
- Weimin Sun (ORCID: https://orcid.org/0000-0001-9300-6425)
- Chao‐Jian Hu (ORCID: https://orcid.org/0009-0002-3566-0917)
- Yuan Yang (ORCID: https://orcid.org/0000-0003-2378-8526)
- Jian‐Yu Jiao (ORCID: https://orcid.org/0000-0002-6900-2296)
- Zheng‐Han Lian (ORCID: https://orcid.org/0000-0002-0562-5849)
- Yu‐Ting OuYang
- Mengmeng Li (ORCID: https://orcid.org/0000-0002-9171-4978)
- Ying Chen (ORCID: https://orcid.org/0000-0001-6396-7815)
- Jianchao Zhang (ORCID: https://orcid.org/0000-0003-4267-1999)
- Lei Gao (ORCID: https://orcid.org/0000-0002-6488-0292)
- Sha Tan (ORCID: https://orcid.org/0000-0002-6891-9502)
- Yu-Xian Li (ORCID: https://orcid.org/0009-0003-5262-6523)
- Zhen‐Hao Luo (ORCID: https://orcid.org/0000-0003-2028-8788)
- Lan Liu (ORCID: https://orcid.org/0000-0002-1433-404X)
Institutions
- University of Science and Technology of China (CN)
- University of Nevada, Las Vegas (US)
- Tianjin University of Technology (CN)
- Tianjin University (CN)
- South China Normal University (CN)
- Chinese Academy of Sciences (CN)
- Institute of Ecology and Geography (MD)
- Xinjiang Institute of Ecology and Geography (CN)
- Guangdong Academy of Sciences (CN)
- Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (CN)
- CAS Key Laboratory of Urban Pollutant Conversion (CN)
- BRIC-National Agri-Food and Biomanufacturing Institute (IN)
- Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (CN)
Publication Details
- Journal
- National Science Review
- Published
- 2024-10-21
- DOI
- https://doi.org/10.1093/nsr/nwae378
- Citations
- 7
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 1.64
Funders
- National Science Foundation
- National Natural Science Foundation of China
- Sun Yat-sen University
- Fundamental Research Funds for the Central Universities