Survey, Collection and In-vitro Study of the Stemphylium Blight Disease in Onion Caused by Stemphylium vesicarium

Onion (Allium cepa L.) is grown all over world and is a favorite vegetable in India. It is relished mostly as salad and Indian cuisine is incomplete without onion. The survey was done during Kharif 2022 and Rabi, 2022-23 at different taluka of Nashik District. At 75 days after transplanting the highest intensity of stemphylium blight (19.2%) was recorded in taluka Deola. The leaves from the affected plant of different age showing the characteristic symptoms of stemphylium blight was brought to the laboratory in polythene bags for critical examination and presence of the pathogen responsible for symptoms production. The best growth of the fungus on solid medium was recorded (86.0mm) in Potato Dextrose Agar medium while, in case of liquid medium the maximum quantitative growth (793.33mg) was recorded in Richard’s medium after 7 days of inoculation. Optimum temperature for the growth of the fungus was 28 °C.