Mitochondrial Genome Assembly and Structural Characteristics Analysis of Gentiana rigescens

<i>Gentiana rigescens</i>, an alpine plant with significant medicinal value, possesses a complex genetic background. However, comprehensive genomic research on <i>G. rigescens</i> is still lacking, particularly concerning its organelle genome. In this study, <i>G. rigescens</i> was studied to sequence the mitochondrial genome (mitogenome) and ascertain the assembly, informational content, and developmental expression of the mitogenome. The mitogenome of <i>G. rigescens</i> was 393,595 bp in length and comprised four circular chromosomes ranging in size from 6646 bp to 362,358 bp. The GC content was 43.73%. The mitogenome featured 30 distinct protein-coding genes, 26 tRNA genes, and 3 rRNA genes. The mitogenome of <i>G. rigescens</i> also revealed 70 SSRs, which were mostly tetra-nucleotides. In addition, 48 homologous fragments were found between the mitogenome and the chloroplast genome, with the longest measuring 23,330 bp. The documentation of the mitochondrial genome of <i>G. rigescens</i> is instrumental in advancing the understanding of its physiological development. Decoding the <i>G. rigescens</i> mitogenome will offer valuable genetic material for phylogenetic research on Gentianaceae and enhance the use of species germplasm resources.