An Optimized <scp>CRISPR</scp>/Cas12a Assay to Facilitate the <scp>BRAF V600E</scp> Mutation Detection
We propose a potential diagnostic approach that is facile, fast, and affordable with high fidelity. This method can detect BRAF V600E mutation with a 2% LOD without the need for a thermocycler.
Authors
- Azadeh Etemadzadeh
- Mohammad Hossein Modarressi (ORCID: https://orcid.org/0000-0003-2763-1964)
- Pouya Salehipour (ORCID: https://orcid.org/0000-0001-8247-0003)
- Adnan Asadbeigi
- Fatemeh Movahedi Motlagh
- Mina Tabrizi (ORCID: https://orcid.org/0000-0002-1677-214X)
- Masoomeh Khalifeh
- Reza Shirkouhi
Institutions
- Mashhad University of Medical Sciences (IR)
- Oregon Health & Science University (US)
- Imam Khomeini Hospital (IR)
- Tehran University of Medical Sciences (IR)
Publication Details
- Journal
- Journal of Clinical Laboratory Analysis
- Published
- 2024-10-24
- DOI
- https://doi.org/10.1002/jcla.25101
- Citations
- 4
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- Tehran University of Medical Sciences and Health Services