Synthesis, <i>In‐Silico</i> Biochemical Properties, and <i>In‐Vitro</i> Antimicrobial Activity of Some Farmazans‐Sulfonamide Derivatives

The low effectiveness of currently available antibiotics is driving efforts worldwide to generate new antimicrobial drugs. So, we synthesized some new formazan derivatives containing sulfonamide moiety, and assessed their in-silico biochemical properties as well as in-vitro antibacterial activity against some pathogenic Gram-positive (B. subtilis and S. aureus) and Gram-negative (E. coli and S. thyphi) bacteria and fungi (C. albicans and A. niger). These formazan derivatives were synthesized by condensing sulphanilamide with benzaldehyde in the presence of glacial acetic acid and ethanol produced a Schiff base of sulfonamide (1). After this compound 1 was reacted with substituted benzene diazonium chlorides (2a-g) by condensation reaction yields formazan derivatives (3a-g). The structures of synthesized compounds were characterized on the basis analytical and spectral (IR, ¹H-NMR, and mass) data. Agar diffusion method was utilized to assess the antibacterial activity of the synthesized compounds by measuring the zone of inhibition against tested strains of bacteria and fungi. Ciprofloxacin and ketoconazole were used as reference drugs. The result exhibited that synthesized compounds have demonstrated satisfactory in-silico biochemical properties as well as significant level of antibacterial activity.