Multi-omics landscape and molecular basis of radiation tolerance in a tardigrade
Tardigrades are captivating organisms known for their resilience in extreme environments, including ultra-high-dose radiation, but the underlying mechanisms of this resilience remain largely unknown. Using genome, transcriptome, and proteome analysis of <i>Hypsibius henanensis</i> <b>sp. nov.</b>, we explored the molecular basis contributing to radiotolerance in this organism. A putatively horizontally transferred gene, DOPA dioxygenase 1 (<i>DODA1</i>), responds to radiation and confers radiotolerance by synthesizing betalains-a type of plant pigment with free radical-scavenging properties. A tardigrade-specific radiation-induced disordered protein, TRID1, facilitates DNA damage repair through a mechanism involving phase separation. Two mitochondrial respiratory chain complex assembly proteins, BCS1 and NDUFB8, accumulate to accelerate nicotinamide adenine dinucleotide (NAD<sup>+</sup>) regeneration for poly(adenosine diphosphate-ribosyl)ation (PARylation) and subsequent poly(adenosine diphosphate-ribose) polymerase 1 (PARP1)-mediated DNA damage repair. These three observations expand our understanding of mechanisms of tardigrade radiotolerance.
Authors
- Zhenzhen Cui
- Baoyu Chen (ORCID: https://orcid.org/0000-0002-6366-159X)
- Junmiao Chen (ORCID: https://orcid.org/0009-0006-5312-456X)
- Inge Seim (ORCID: https://orcid.org/0000-0001-8594-7217)
- Xiaoguang Lei (ORCID: https://orcid.org/0000-0002-0380-8035)
- Cui Hua Liu (ORCID: https://orcid.org/0000-0002-8035-7792)
- Pan Shen (ORCID: https://orcid.org/0000-0003-3374-0382)
- Lingqiang Zhang (ORCID: https://orcid.org/0000-0002-9795-2141)
- Yishan Yao (ORCID: https://orcid.org/0000-0002-8492-1688)
- Mu Duan (ORCID: https://orcid.org/0009-0009-9315-9304)
- Guangyi Fan (ORCID: https://orcid.org/0000-0001-7365-1590)
- Maojun Yang (ORCID: https://orcid.org/0000-0002-6798-3094)
- Liangwei Li (ORCID: https://orcid.org/0000-0002-9315-4353)
- Ruochong Li (ORCID: https://orcid.org/0000-0001-7372-3267)
- Chaoran Li (ORCID: https://orcid.org/0009-0005-1034-3378)
- Yesheng Fu (ORCID: https://orcid.org/0000-0002-3947-601X)
- Kaixin Chen (ORCID: https://orcid.org/0000-0001-7338-8032)
- Arong Luo (ORCID: https://orcid.org/0000-0001-9652-5896)
- Zhixiang Yang
- Xin Liu (ORCID: https://orcid.org/0000-0003-3256-2940)
- Lei Li (ORCID: https://orcid.org/0000-0002-3930-8809)
- Jun Chen (ORCID: https://orcid.org/0000-0002-1273-5624)
- Fei Wang (ORCID: https://orcid.org/0000-0001-5297-0859)
- Lizhi Wang (ORCID: https://orcid.org/0009-0002-8543-3229)
- Yaqi Li
- Shihao Liu (ORCID: https://orcid.org/0009-0000-6092-5724)
- Dong Yang (ORCID: https://orcid.org/0000-0002-1717-6833)
- Guibin Wang (ORCID: https://orcid.org/0000-0001-9911-067X)
- Fusheng Guo (ORCID: https://orcid.org/0009-0009-5601-2661)
- Kun Zheng (ORCID: https://orcid.org/0000-0003-3379-3003)
- Jin Huang (ORCID: https://orcid.org/0009-0006-8401-7855)
- Yudie Ning
- Zimei Sun (ORCID: https://orcid.org/0009-0007-9023-2452)
- Z. W. Ge (ORCID: https://orcid.org/0009-0007-8633-2549)
- Yifan Wang (ORCID: https://orcid.org/0009-0006-1265-4361)
- Yanyan Liu (ORCID: https://orcid.org/0009-0004-2194-8017)
Institutions
- Beijing Proteome Research Center (CN)
- Institute of Deep-Sea Science and Engineering (CN)
Publication Details
- Journal
- Science
- Published
- 2024-10-24
- DOI
- https://doi.org/10.1126/science.adl0799
- Citations
- 39
- Primary Topic
- Tardigrade Biology and Ecology
- Type
- article
- Field-Weighted Citation Impact
- 40.13