Effect of bacterial dissociation on lipopolysaccharide structure: A study of O-polysaccharide from the marine bacterium Pseudoalteromonas agarivorans KMM 232 (O-form)
The lipopolysaccharide (LPS) was obtained from a bacterium Pseudoalteromonas agarivorans KMM 232 (O-form) isolated from a seawater sample collected at a depth of 500 m. The O-polysaccharide (OPS) was isolated by mild acid degradation of the LPS and studied by chemical methods along with 1D and 2D <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy, including <sup>1</sup>H,<sup>1</sup>H COSY, <sup>1</sup>H,<sup>1</sup>H TOCSY, <sup>1</sup>H,<sup>1</sup>H ROESY and <sup>1</sup>H,<sup>13</sup>C HSQC, and <sup>1</sup>H,<sup>13</sup>C HMBC experiments. The following new structure of the OPS from P. agarivorans KMM 232 (O-form) containing 2-acetamido-2-deoxy-d-glucose (D-GlcNAc), d-glucose (D-Glc), d-glucuronic acid (D-GlcA), 4,6-O-[(R)-1-carboxyethylidene]-d-galactose [D-Galp4,6 (R-Pyr)] and two residues of d-galactose (D-Gal) was established.
Authors
- Vlada S Belova (ORCID: https://orcid.org/0000-0001-9174-0220)
- Lyudmila A. Romanenko (ORCID: https://orcid.org/0000-0001-9843-3637)
- Maxim S. Kokoulin (ORCID: https://orcid.org/0000-0003-2245-6802)
Institutions
- Pacific Institute of Bioorganic Chemistry. GB Elyakova Far Eastern Branch of the Russian Academy of Sciences (RU)
Publication Details
- Journal
- Carbohydrate Research
- Published
- 2024-10-24
- DOI
- https://doi.org/10.1016/j.carres.2024.109300
- Citations
- 1
- Primary Topic
- Carbohydrate Chemistry and Synthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.25