Effect of Macronutrients on Recombinant mCherry Production by Microalga

Abstract This study sought to evaluate the influence of five macronutrients (acetate, calcium, sulfate, nitrogen, and phosphate) on the cell growth and heterologous m Cherry protein production by Chlamydomonas reinhardtii . In the first step, three nitrogen (N) sources were tested (NH 4 NO 3 , NaNO 3 , and NH 4 Cl), and NH 4 NO 3 was selected as N source for the following step, due to the best results for m Cherry protein production. In the second step, a central composite design 2 5 was employed to evaluate the effect of the five macronutrients. Although all nutrients had effect on maximum biomass concentration, only acetate, nitrogen, and phosphate showed to influence m Cherry protein production. By employing the experimental design with small‐scale culture in multiplates and multivariable regression, it was possible to achieve 12 % increase for recombinant protein production.