A single-stranded based library preparation method for virome characterization
We introduce and improve a fast, simple, and efficient ligation-based single-stranded DNA library preparation for gut virome study. This method is compatible with Illumina sequencing platforms and only requires ligation reagents within 3-h library preparation, which is similar or even better than the advanced library preparation method (xGen). We hope this method can be further optimized, validated, and widely used to make gut virome study more comparable and reproducible. Video Abstract.
Authors
- Xichuan Zhai (ORCID: https://orcid.org/0000-0002-7498-1230)
- Łukasz Krych (ORCID: https://orcid.org/0000-0003-3224-1346)
- Witold Kot (ORCID: https://orcid.org/0000-0002-0188-4587)
- Alex Gobbi (ORCID: https://orcid.org/0000-0003-3541-4732)
- Ling Deng (ORCID: https://orcid.org/0000-0002-2190-9788)
- Dennis Sandris Nielsen (ORCID: https://orcid.org/0000-0001-8121-1114)
Institutions
- University of Copenhagen (DK)
- Danish Ministry of Food, Agriculture and Fisheries (DK)
- University of Turin (IT)
Publication Details
- Journal
- Microbiome
- Published
- 2024-10-24
- DOI
- https://doi.org/10.1186/s40168-024-01935-5
- Citations
- 5
- Primary Topic
- Bacteriophages and microbial interactions
- Type
- article
- Field-Weighted Citation Impact
- 2.29
Funders
- Villum Fonden
- Københavns Universitet
- Eidgenössische Technische Hochschule Zürich
- China Scholarship Council