Synthesis of Carboxylate-Dialdehyde Cellulose to Use as a Component in Composite Thin Films for an Antibacterial Material in Wound Dressing

Wound infections can lead to life-threatening infection and death. Antibacterial materials from biopolymers in the form of films are a promising strategy for wound dressings. Carboxylate-dialdehyde cellulose (CDAC) is a proper candidate for use as an antibacterial material due to its biocompatibility, nontoxicity, and antibacterial property. Additionally, CDAC can be synthesized from cellulose through environmentally friendly and nontoxic methods. Thus, this study aims to synthesize CDAC from microcrystalline cellulose (MCC) PH102 and use it in composite films for an antibacterial application. The CDAC was synthesized using Fe²⁺/H₂O₂, followed by NaIO₄ oxidation. The obtained CDAC was characterized in terms of carboxylate and aldehyde content as well as FTIR and XRD spectra. The CDAC was mixed with HPMC in different ratios to prepare films. To determine the optimal formulation for clindamycin HCl loading, the films were evaluated for morphology, mechanical properties, and swelling ratio. Finally, the films containing clindamycin HCl were evaluated for drug loading content, <i>in vitro</i> drug release, and antibacterial activity. This study found that CDAC contained 2.1 ± 0.2 carboxylate and 4.15 ± 0.2 mmol/g of aldehyde content. The FTIR spectra confirmed the successful synthesis. X-ray diffractograms indicated that CDAC was less crystalline than MCC. The film, consisting of CDAC and HPMC E50 in the ratio of 2:1 (D2H1), was identified as the most suitable for clindamycin HCl loading due to its superior appearance, mechanical strength, and swelling properties compared to other formulations. D2H1 exhibited a high drug loading capacity (91.49 ± 5.48%) and demonstrated faster drug release than the film composed only of HPMC because of the higher swelling ratio and lower mechanical strength. This formulation was effective against <i>Staphylococcus aureus</i> (MSSA), <i>S. aureus</i> (MRSA), and <i>Pseudomonas aeruginosa</i>. Furthermore, the D2H1 film containing clindamycin HCl showed a larger inhibition zone against these bacteria, likely due to a synergistic effect. This study found that CDAC has the potential to be applied as an antibacterial material for wound dressing.