Exploring the Siderophore Portfolio for Mass Spectrometry-Based Diagnosis of Scedosporiosis and Lomentosporiosis
<i>Scedosporium apiospermum</i> and <i>Lomentospora prolificans</i> secrete siderophores (iron scavengers) during hyphal proliferation. Siderophores are virulence factors and potential clinical biomarkers of invasive scedosporiosis and lomentosporiosis. Both strains secreted a uniform spectrum of siderophores, including coprogen B (CopB), <i>N</i> <sup>α</sup>-methyl-coprogen B, dimethyl-coprogen, and ferricrocin, with <i>N</i> <sup>α</sup>-methyl-coprogen B being the fastest secreted and most abundant coprogen. Under iron and zinc restriction, reflecting a nutrient-limited host environment, <i>L. prolificans</i> secreted 45 times more CopB than did <i>S. apiospermum</i>, presumably contributing to its higher virulence. This robust mobilization of CopB was further enhanced by zinc surplus. Additionally, two novel cyclic peptides, Scedocyclin A and B, were characterized in<i>Scedosporium boydii</i> using the <i>de novo</i> sequencing tool CycloBranch. Utilizing matrix-assisted laser desorption/ionization, the portfolio of coprogens detected had limits of detection and quantitation of 4.9 and 14.6 fmol/spot in complex matrices, respectively, making them strong candidates for the next-generation, routine diagnosis of invasive scedosporiosis and lomentosporiosis through the Biotyper siderotyping.
Authors
- Radim Dobiáš (ORCID: https://orcid.org/0000-0003-4299-9169)
- Helena Marešová (ORCID: https://orcid.org/0000-0003-2462-390X)
- Tomáš Pluháček (ORCID: https://orcid.org/0000-0002-7417-0016)
- David A. Stevens (ORCID: https://orcid.org/0000-0002-5861-5716)
- Hélène Guegan (ORCID: https://orcid.org/0000-0001-6338-3094)
- Jiří Houšť (ORCID: https://orcid.org/0000-0002-3754-6539)
- Jiří Novák (ORCID: https://orcid.org/0000-0003-1902-4304)
- Petr Hubáček (ORCID: https://orcid.org/0000-0002-6030-2604)
- Jean‐Pierre Gangneux (ORCID: https://orcid.org/0000-0002-4974-5607)
- Vladimı́r Havlı́ček (ORCID: https://orcid.org/0000-0002-8614-7059)
- Andrea Palyzová (ORCID: https://orcid.org/0000-0003-0852-9841)
Institutions
- Inserm (FR)
- University of Ostrava (CZ)
- Czech Academy of Sciences (CZ)
- Charles University (CZ)
- University Hospital in Motol (CZ)
- Public Health Institute Ostrava (CZ)
- Institut de Recherche en Santé, Environnement et Travail (FR)
- National Foundation for Infectious Diseases (US)
- Czech Academy of Sciences, Institute of Microbiology (CZ)
- Centre Hospitalier Universitaire de Rennes (FR)
- Czech Technical University in Prague (CZ)
- Palacký University Olomouc (CZ)
- Stanford University (US)
Publication Details
- Journal
- ACS Omega
- Published
- 2024-10-23
- DOI
- https://doi.org/10.1021/acsomega.4c08257
- Citations
- 1
- Primary Topic
- Parasitic Infections and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 0.52
Funders
- Univerzita Karlova v Praze
- České Vysoké Učení Technické v Praze
- Institut National de la Santé et de la Recherche Médicale
- Ministerstvo Školství, Mládeže a Tělovýchovy
- Akademie Věd České Republiky
- Ostravská Univerzita v Ostravě
- Univerzita Palackého v Olomouci
- Centre Hospitalier Universitaire de Rennes
- School of Medicine, Stanford University
- Mikrobiologický Ústav, Akademie Věd České Republiky