Sleep Potentiating Effect of <i>Pleurotus squarrusolus</i> Ethanolic Extract on Diazepam-Induced Sleeping Time in Male Wistar Rats

This study examines the sleep-promoting properties of an ethanolic extract derived from <i>Pleurotus squarrosulus</i> in male Wistar rats, particularly in conjunction with the sleep-inducing effects of diazepam. The impetus for this research is driven by the rising global incidence of sleep disorders and the ongoing need for effective, natural adjuncts to current therapeutic strategies. The bioactive compounds identified in <i>Pleurotus squarrosulus</i> may exert mood-modulating effects, which could play a role in its overall therapeutic impact on sleep and well-being. The extract was subjected to analysis using High-Performance Liquid Chromatography (HPLC), which revealed the presence of gallic acid, p-coumaric acid, ferulic acid, catechin, rutin, apigenin, quercetin, and kaempferol. Among these compounds, gallic acid and quercetin exhibited the highest concentrations. Diazepam, a traditional sedative, was administered alone as well as in combination with two different doses of the extract, namely 250mg and 500mg. The results demonstrated a significant increase in sleeping time in both the Diazepam + Sample 250mg and Diazepam + Sample 500mg groups compared to the control group, indicating an enhancement in sleep quality. These findings are consistent with previous research, which highlighted the presence of bioactive compounds known for their diverse biological effects. This study introduces <i>Pleurotus squarrosulus </i>as a potential natural sleep aid, showcasing a promising synergy when combined with diazepam. Further investigation into the mechanisms and safety considerations of this combination offers an optimistic path for the development of integrated approaches to sleep management.