Triplex real-time PCR ZKIR-T assay for simultaneous detection of the <i>Klebsiella pneumoniae</i> species complex and identification of <i>K. pneumoniae</i> sensu stricto
The pathogens of the <i>Klebsiella pneumoniae</i> species complex are widespread in food and animals and are among the main pathogens responsible for multidrug resistant infections in humans. In this study, we developed a highly sensitive detection assay that enables detection of this group of bacteria, with the simultaneous identification of the most common and clinically important species. This triplex one-reaction assay was shown to be highly sensitive and precise, enabling fast screening of varied samples for the presence of KpSC and <i>K. pneumoniae</i> sensu stricto.
Authors
- Alain Hartmann (ORCID: https://orcid.org/0000-0001-6034-5068)
- Carla Rodrigues (ORCID: https://orcid.org/0000-0002-9608-2404)
- Sylvain Brisse (ORCID: https://orcid.org/0000-0002-2516-2108)
- Eva Møller Nielsen
- Elodie Barbier
- Małgorzata Ligowska-Marzęta (ORCID: https://orcid.org/0000-0002-2693-5141)
- Dennis Schrøder Hansen (ORCID: https://orcid.org/0000-0002-8256-2028)
- Pascal Piveteau (ORCID: https://orcid.org/0000-0002-1210-251X)
Institutions
- Statens Serum Institut (DK)
- Institut Pasteur (FR)
- Université de Bourgogne (FR)
- Université Paris Cité (FR)
- Copenhagen University Hospital (DK)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Agroécologie (FR)
- Institut Agro Dijon (FR)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2024-10-22
- DOI
- https://doi.org/10.1128/spectrum.00336-24
- Citations
- 4
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.68
Funders
- European Commission
- Danmarks Tekniske Universitet