IL-1β-induced epithelial cell and fibroblast transdifferentiation promotes neutrophil recruitment in chronic rhinosinusitis with nasal polyps
Neutrophilic inflammation contributes to multiple chronic inflammatory airway diseases, including asthma and chronic rhinosinusitis with nasal polyps (CRSwNP), and is associated with an unfavorable prognosis. Here, using single-cell RNA sequencing (scRNA-seq) to profile human nasal mucosa obtained from the inferior turbinates, middle turbinates, and nasal polyps of CRSwNP patients, we identify two IL-1 signaling-induced cell subsets-LY6D<sup>+</sup> club cells and IDO1<sup>+</sup> fibroblasts-that promote neutrophil recruitment by respectively releasing S100A8/A9 and CXCL1/2/3/5/6/8 into inflammatory regions. IL-1β, a pro-inflammatory cytokine involved in IL-1 signaling, induces the transdifferentiation of LY6D<sup>+</sup> club cells and IDO1<sup>+</sup> fibroblasts from primary epithelial cells and fibroblasts, respectively. In an LPS-induced neutrophilic CRSwNP mouse model, blocking IL-1β activity with a receptor antagonist significantly reduces the numbers of LY6D<sup>+</sup> club cells and IDO1<sup>+</sup> fibroblasts and mitigates nasal inflammation. This study implicates the function of two cell subsets in neutrophil recruitment and demonstrates an IL-1-based intervention for mitigating neutrophilic inflammation in CRSwNP.
Authors
- Pin Wang (ORCID: https://orcid.org/0000-0002-4457-1695)
- Changhua Wu (ORCID: https://orcid.org/0000-0001-6206-557X)
- Xuezhong Li (ORCID: https://orcid.org/0000-0001-7482-7930)
- Xin Feng (ORCID: https://orcid.org/0000-0001-8089-4005)
- Fangying Liu
- Xinyu Xie (ORCID: https://orcid.org/0000-0001-5039-3349)
- Shu Guo (ORCID: https://orcid.org/0000-0001-9094-1602)
- Min Jin (ORCID: https://orcid.org/0000-0002-1390-4549)
- Larry Borish (ORCID: https://orcid.org/0000-0002-8298-8184)
- Weiyuan Liu (ORCID: https://orcid.org/0000-0002-5376-0180)
- Xiaojun Zhang (ORCID: https://orcid.org/0000-0002-4559-0177)
- Heng Liu (ORCID: https://orcid.org/0000-0001-6683-1462)
- Wei Zhao (ORCID: https://orcid.org/0000-0001-8440-2274)
- Yue Wang (ORCID: https://orcid.org/0000-0003-2486-6071)
- Changqing Li (ORCID: https://orcid.org/0000-0003-4380-2284)
- Ping Ye (ORCID: https://orcid.org/0000-0003-1408-8704)
- Xinyi Ma
- Ye Yuan (ORCID: https://orcid.org/0000-0001-9628-6944)
- Lijie Qi
- Chen Duan (ORCID: https://orcid.org/0000-0002-6223-9792)
Institutions
- Shandong University (CN)
- University of Virginia Health System (US)
- Qilu Hospital of Shandong University (CN)
Publication Details
- Journal
- Nature Communications
- Published
- 2024-10-22
- DOI
- https://doi.org/10.1038/s41467-024-53307-0
- Citations
- 33
- Primary Topic
- Sinusitis and nasal conditions
- Type
- article
- Field-Weighted Citation Impact
- 19.13
Funders
- Taishan Scholar Foundation of Shandong Province
- National Natural Science Foundation of China
- Natural Science Foundation of Shandong Province