Preparation and Characterization of Nypa Fruit Pulp Extraction as a Natural Material for Scaffold Fabrication using 3D Printing Method

The aim of this study is to investigate the extracted Nypa fruticans as an alternative biomaterial used for 3D printing application in the biomedical and pharmaceutical. An important substances that was extracted from nipa palm fruit is galactomannan, a water-soluble heteropolysaccharide. It was considered to the polysaccharide-based polymer material used to fabricate scaffold tissue by 3D printing approach for medical purposes. Emphasis was placed on the synthesis and characterization of extracted nypa fruticans as the primary material, along with glycerol and sorbitol serving as polimerization agents. Extracted nypa with 3%, 5% and 10% amout of variation glycerol and sorbitol was evaluated. The experimental procedures conducted in the laboratory facilitated the isolation, production, purification, and fabrication of the extracted nypa polymer gels. Various properties of the nypa compounds and chemical composition through Fourier-transform infrared (FTIR) Spectrometry were analyzed. Findings indicated that extracted nypa refer to ether bonds (CH2-O- CH2) observed at $875.04 \mathbf{c m}^{-1}$, identifying compound is galactomannan. This substance is classified within the polysaccharide category due to the appearance of peaks within the range of 900 to $1200 \mathrm{~cm}^{-1}$. The incorporation of different amount percentage of glycerol and sorbitol, resulted in distinct impacts on the geometry of 3D printing scaffold material laying down.