Urine organic acid metabolomic profiling by gas chromatography mass spectrometry: Assessment of solvent extract evaporation parameters on the recovery of key diagnostic metabolites
Evaporation of solvent extracts with heat and/or prolonged drying under nitrogen results in significant losses of the shorter chain hydroxycarboxylic acids. The evaporation protocol must be carefully controlled to ensure accurate and reproducible results, preventing misdiagnoses and/or misinterpretation of results.
Authors
- Stuart J. Moat (ORCID: https://orcid.org/0000-0002-3592-2254)
- Rachel S. Carling (ORCID: https://orcid.org/0000-0003-1988-3088)
- Erin C. Emmett
- Claire Joanne Gallagher (ORCID: https://orcid.org/0009-0001-3938-5441)
- Karolina Witek
Institutions
- St Thomas' Hospital (GB)
- University Hospital of Wales (GB)
Publication Details
- Journal
- Clinica Chimica Acta
- Published
- 2024-10-22
- DOI
- https://doi.org/10.1016/j.cca.2024.120015
- Citations
- 1
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- Medical Research Council