Cu-Albumin Artificial Enzymes with Peroxidase and Oxidase Activity for Stereoselective Oxidations
We herein report a design of artificial enzymes by incorporating a synthetic copper complex into noncatalytic bovine serum albumin (Cu-BSA) to carry out stereoselective oxidation. This Cu-BSA catalyst with stably bound Cu complex as a cofactor shows peroxidase-like activity to catalyze epoxidation of styrene with high chiral selectivity (>99%) to R-styrene epoxide. With the electrochemical conversion of Cu2+ to Cu+, Cu-BSA also exhibits oxidase-like activity to selectively reduce oxygen to hydrogen peroxide (H2O2), which can be combined with its peroxidase function to drive oxidation of C═C bonds using air. This artificial enzymatic system holds promise for chiral-selective transformations of non-natural substances and highlights the versatility of noncatalytic proteins in the design of artificial enzymes.
Authors
- Challa V. Kumar (ORCID: https://orcid.org/0000-0002-8399-1334)
- Jie He (ORCID: https://orcid.org/0000-0003-0252-3094)
- E. McDonald (ORCID: https://orcid.org/0009-0000-2838-4905)
- Maham Liaqat (ORCID: https://orcid.org/0000-0003-0506-7055)
- James F. Rusling (ORCID: https://orcid.org/0000-0002-6117-3306)
- Xudong Yao (ORCID: https://orcid.org/0000-0001-9432-5065)
- Aaron Lopes
- Rocio Ortega
- Flavia Codreanu
- Hannah Carlisle
Institutions
- Brigham and Women's Hospital (US)
- Northeastern University (US)
- Harvard University (US)
- University of Connecticut (US)
- University of Toronto (CA)
- Ollscoil na Gaillimhe – University of Galway (IE)
- Massachusetts Institute of Technology (US)
- UConn Health (US)
- Universidad del Noreste (MX)
Publication Details
- Journal
- ACS Catalysis
- Published
- 2024-10-22
- DOI
- https://doi.org/10.1021/acscatal.4c05732
- Citations
- 8
- Primary Topic
- Advanced Nanomaterials in Catalysis
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- University of Connecticut
- Division of Chemical, Bioengineering, Environmental, and Transport Systems